Structural insights into binding of STAC proteins to voltage-gated calcium channels.

Excitation-contraction (EC) coupling in skeletal muscle requires purposeful and mechanical coupling between L-type voltage-gated calcium channels (CaV1.1) and the ryanodine receptor (RyR1). Recently, STAC3 was recognized as an important protein for EC coupling and is an element of a gaggle of three proteins that may bind and modulate L-type voltage-gated calcium channels.

Here, we report crystal constructions of tandem-SH3 domains of completely different STAC isoforms up to 1.2-Å decision. These type a inflexible interplay by means of a conserved interdomain interface. We determine the linker connecting transmembrane repeats II and III in two completely different CaV isoforms as a binding website for the SH3 domains and report a crystal construction of the complicated with the STAC2 isoform.

The interplay website contains the placement for a illness variant in STAC3 that has been linked to Native American myopathy (NAM). Introducing the mutation doesn’t trigger misfolding of the SH3 domains, however abolishes the interplay. Disruption of the interplay through mutations within the II-III loop perturbs skeletal muscle EC coupling, however preserves the flexibility of STAC3 to decelerate inactivation of CaV1.2.

Calcium waves in a grid of clustered channels with synchronous IP3 binding and unbinding.

Calcium indicators in cells happen at a number of spatial scales and variable temporal length. However, a bodily rationalization for transitions between long-lasting international oscillations and localized short-term elevations (puffs) of cytoplasmic Ca2+ remains to be missing.

Here we introduce a phenomenological, coarse-grained mannequin for the calcium variable, which is represented by bizarre differential equations. Due to its small quantity of parameters, and its simplicity, this mannequin permits us to numerically research the interaction of multi-scale calcium concentrations with stochastic ion channel gating dynamics even in bigger techniques.

ANK1 siRNA
20-abx907484	Abbexa	661.20 EUR 878.40 EUR	15 nmol 30 nmol
ANK1 cloning plasmid
CSB-CL001720HU1-10ug	Cusabio	10ug	279.6 EUR
Description: A cloning plasmid for the ANK1 gene.
ANK1 cloning plasmid
CSB-CL001720HU2-10ug	Cusabio	10ug	279.6 EUR
Description: A cloning plasmid for the ANK1 gene.
Human ANK1 shRNA Plasmid
20-abx950199	Abbexa	961.20 EUR 1345.20 EUR	150 µg 300 µg
Mouse ANK1 shRNA Plasmid
20-abx969140	Abbexa	961.20 EUR 1345.20 EUR	150 µg 300 µg
Ankyrin 1 (ANK1) Antibody
abx037600-100ug	Abbexa	100 ug	469.2 EUR
Ankyrin 1 (ANK1) Antibody
abx430864-200ul	Abbexa	200 ul	343.2 EUR
Ankyrin R (ANK1) Antibody
abx445096-100ug	Abbexa	100 ug	627.6 EUR
ANK1 ELISA KIT|Human
EF004617	Lifescience Market	96 Tests	826.8 EUR
Ankyrin 1, Erythrocytic (ANK1) Antibody
20-abx128802	Abbexa	493.20 EUR 159.60 EUR 1378.80 EUR 678.00 EUR 376.80 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Ankyrin R (ANK1) Antibody (APC)
abx442774-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (Biotin)
abx443054-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (FITC)
abx443334-100ug	Abbexa	100 ug	678 EUR
Ankyrin R (ANK1) Antibody (HRP)
abx443615-100ug	Abbexa	100 ug	678 EUR
Ankyrin R (ANK1) Antibody (PerCP)
abx444177-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (RPE)
abx444458-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (Streptavidin)
abx444739-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (ALP)
abx442493-100ug	Abbexa	100 ug	693.6 EUR
Polyclonal ANK1 antibody - middle region
APR00810G	Leading Biology	0.05mg	633.6 EUR
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ANK1 - middle region. This antibody is tested and proven to work in the following applications:
Ank1 ORF Vector (Rat) (pORF)
ORF063392	ABM	1.0 ug DNA	2496 EUR
ANK1 ORF Vector (Human) (pORF)
ORF000405	ABM	1.0 ug DNA	114 EUR
ANK1 ORF Vector (Human) (pORF)
ORF000406	ABM	1.0 ug DNA	114 EUR
Ank1 ORF Vector (Mouse) (pORF)
ORF038594	ABM	1.0 ug DNA	1886.4 EUR
Ank1 ORF Vector (Mouse) (pORF)
ORF038595	ABM	1.0 ug DNA	1886.4 EUR
Anti-Ankyrin 1 / ANK1 antibody
STJ71553	St John's Laboratory	100 µg	312 EUR
Recombinant Ankyrin 1, Erythrocytic (ANK1)
4-RPB633Hu01	Cloud-Clone	571.58 EUR 276.00 EUR 1813.44 EUR 684.48 EUR 1248.96 EUR 458.40 EUR 4353.60 EUR	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Ankyrin 1, Erythrocytic expressed in: E.coli
Human Ankyrin 1(ANK1) ELISA kit
E01A1448-192T	BlueGene	192 tests	1524 EUR
Description: A sandwich ELISA for quantitative measurement of Human Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Ankyrin 1(ANK1) ELISA kit
E01A1448-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A sandwich ELISA for quantitative measurement of Human Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Ankyrin 1(ANK1) ELISA kit
E01A1448-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A sandwich ELISA for quantitative measurement of Human Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Ankyrin 1(ANK1) ELISA kit
E02A1448-192T	BlueGene	192 tests	1524 EUR
Description: A sandwich ELISA for quantitative measurement of Rat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Ankyrin 1(ANK1) ELISA kit
E02A1448-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A sandwich ELISA for quantitative measurement of Rat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Ankyrin 1(ANK1) ELISA kit
E02A1448-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A sandwich ELISA for quantitative measurement of Rat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Ankyrin 1(ANK1) ELISA kit
E07A1448-192T	BlueGene	192 tests	1524 EUR
Description: A sandwich ELISA for quantitative measurement of Porcine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Ankyrin 1(ANK1) ELISA kit
E07A1448-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A sandwich ELISA for quantitative measurement of Porcine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Ankyrin 1(ANK1) ELISA kit
E07A1448-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A sandwich ELISA for quantitative measurement of Porcine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Ankyrin 1(ANK1) ELISA kit
E04A1448-192T	BlueGene	192 tests	1524 EUR
Description: A sandwich ELISA for quantitative measurement of Rabbit Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Ankyrin 1(ANK1) ELISA kit
E04A1448-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A sandwich ELISA for quantitative measurement of Rabbit Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Ankyrin 1(ANK1) ELISA kit
E04A1448-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A sandwich ELISA for quantitative measurement of Rabbit Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Ankyrin 1(ANK1) ELISA kit
E08A1448-192T	BlueGene	192 tests	1524 EUR
Description: A sandwich ELISA for quantitative measurement of Canine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Ankyrin 1(ANK1) ELISA kit
E08A1448-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A sandwich ELISA for quantitative measurement of Canine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Ankyrin 1(ANK1) ELISA kit
E08A1448-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A sandwich ELISA for quantitative measurement of Canine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Ankyrin 1(ANK1) ELISA kit
E09A1448-192T	BlueGene	192 tests	1524 EUR
Description: A sandwich ELISA for quantitative measurement of Monkey Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Ankyrin 1(ANK1) ELISA kit
E09A1448-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A sandwich ELISA for quantitative measurement of Monkey Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Ankyrin 1(ANK1) ELISA kit
E09A1448-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A sandwich ELISA for quantitative measurement of Monkey Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Ankyrin 1(ANK1) ELISA kit
E06A1448-192T	BlueGene	192 tests	1524 EUR
Description: A sandwich ELISA for quantitative measurement of Goat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Ankyrin 1(ANK1) ELISA kit
E06A1448-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A sandwich ELISA for quantitative measurement of Goat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Ankyrin 1(ANK1) ELISA kit
E06A1448-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A sandwich ELISA for quantitative measurement of Goat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Ankyrin 1(ANK1) ELISA kit
E03A1448-192T	BlueGene	192 tests	1524 EUR
Description: A sandwich ELISA for quantitative measurement of Mouse Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Ankyrin 1(ANK1) ELISA kit
E03A1448-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A sandwich ELISA for quantitative measurement of Mouse Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Ankyrin 1(ANK1) ELISA kit
E03A1448-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A sandwich ELISA for quantitative measurement of Mouse Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Ankyrin 1, Erythrocytic (ANK1) Protein
20-abx168126	Abbexa	794.40 EUR 326.40 EUR 2448.00 EUR 944.40 EUR 577.20 EUR	100 ug 10 ug 1 mg 200 ug 50 ug
Human Ankyrin 1 (ANK1) ELISA Kit
abx384586-96tests	Abbexa	96 tests	1093.2 EUR
Mouse Ankyrin 1 (ANK1) ELISA Kit
abx388583-96tests	Abbexa	96 tests	1093.2 EUR
Ankyrin R (ANK1) Antibody (ATTO 390)
abx440245-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (ATTO 488)
abx440526-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (ATTO 565)
abx440807-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (ATTO 594)
abx441088-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (ATTO 633)
abx441369-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (ATTO 655)
abx441650-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (ATTO 680)
abx441931-100ug	Abbexa	100 ug	693.6 EUR
Ankyrin R (ANK1) Antibody (ATTO 700)
abx442212-100ug	Abbexa	100 ug	693.6 EUR
Ank1 sgRNA CRISPR Lentivector set (Mouse)
K3878001	ABM	3 x 1.0 ug	406.8 EUR
Mouse Ankyrin- 1, Ank1 ELISA KIT
ELI-49245m	Lifescience Market	96 Tests	1038 EUR
Human Ankyrin- 1, ANK1 ELISA KIT
ELI-49312h	Lifescience Market	96 Tests	988.8 EUR
ANK1 sgRNA CRISPR Lentivector set (Human)
K0086301	ABM	3 x 1.0 ug	406.8 EUR
Ank1 sgRNA CRISPR Lentivector set (Rat)
K6307301	ABM	3 x 1.0 ug	406.8 EUR
Guinea pig Ankyrin 1(ANK1) ELISA kit
E05A1448-192T	BlueGene	192 tests	1524 EUR
Description: A sandwich ELISA for quantitative measurement of Guinea pig Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Ankyrin 1(ANK1) ELISA kit
E05A1448-48	BlueGene	1 plate of 48 wells	624 EUR
Description: A sandwich ELISA for quantitative measurement of Guinea pig Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Ankyrin 1(ANK1) ELISA kit
E05A1448-96	BlueGene	1 plate of 96 wells	822 EUR
Description: A sandwich ELISA for quantitative measurement of Guinea pig Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Ankyrin R (ANK1) Antibody (PE/ATTO 594)
abx443896-100ug	Abbexa	100 ug	710.4 EUR
Polyclonal Ankyrin 1 / ANK1 Antibody (C-Term)
APG00534G	Leading Biology	0.1mg	580.8 EUR
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Ankyrin 1 / ANK1 (C-Term). This antibody is tested and proven to work in the following applications:
Ank1 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K3878002	ABM	1.0 ug DNA	184.8 EUR
Ank1 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K3878003	ABM	1.0 ug DNA	184.8 EUR
Ank1 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K3878004	ABM	1.0 ug DNA	184.8 EUR
ANK1 sgRNA CRISPR Lentivector (Human) (Target 1)
K0086302	ABM	1.0 ug DNA	184.8 EUR
ANK1 sgRNA CRISPR Lentivector (Human) (Target 2)
K0086303	ABM	1.0 ug DNA	184.8 EUR
ANK1 sgRNA CRISPR Lentivector (Human) (Target 3)
K0086304	ABM	1.0 ug DNA	184.8 EUR
Ank1 sgRNA CRISPR Lentivector (Rat) (Target 1)
K6307302	ABM	1.0 ug DNA	184.8 EUR
Ank1 sgRNA CRISPR Lentivector (Rat) (Target 2)
K6307303	ABM	1.0 ug DNA	184.8 EUR
Ank1 sgRNA CRISPR Lentivector (Rat) (Target 3)
K6307304	ABM	1.0 ug DNA	184.8 EUR
ANK1 Protein Vector (Human) (pPB-C-His)
PV001617	ABM	500 ng	394.8 EUR
ANK1 Protein Vector (Human) (pPB-N-His)
PV001618	ABM	500 ng	394.8 EUR
ANK1 Protein Vector (Human) (pPM-C-HA)
PV001619	ABM	500 ng	394.8 EUR
ANK1 Protein Vector (Human) (pPM-C-His)
PV001620	ABM	500 ng	394.8 EUR
ANK1 Protein Vector (Human) (pPB-C-His)
PV001621	ABM	500 ng	394.8 EUR
ANK1 Protein Vector (Human) (pPB-N-His)
PV001622	ABM	500 ng	394.8 EUR
ANK1 Protein Vector (Human) (pPM-C-HA)
PV001623	ABM	500 ng	394.8 EUR
ANK1 Protein Vector (Human) (pPM-C-His)
PV001624	ABM	500 ng	394.8 EUR
ANK1 Protein Vector (Mouse) (pPB-C-His)
PV154374	ABM	500 ng	3681.6 EUR
ANK1 Protein Vector (Mouse) (pPB-N-His)
PV154375	ABM	500 ng	3681.6 EUR
ANK1 Protein Vector (Mouse) (pPM-C-HA)
PV154376	ABM	500 ng	3681.6 EUR
ANK1 Protein Vector (Mouse) (pPM-C-His)
PV154377	ABM	500 ng	3681.6 EUR
ANK1 Protein Vector (Mouse) (pPB-C-His)
PV154378	ABM	500 ng	3788.4 EUR
ANK1 Protein Vector (Mouse) (pPB-N-His)
PV154379	ABM	500 ng	3788.4 EUR
ANK1 Protein Vector (Mouse) (pPM-C-HA)
PV154380	ABM	500 ng	3788.4 EUR
ANK1 Protein Vector (Mouse) (pPM-C-His)
PV154381	ABM	500 ng	3788.4 EUR
Ankyrin 1, Erythrocytic (ANK1) Polyclonal Antibody (Human)
4-PAB633Hu01	Cloud-Clone	286.80 EUR 2869.20 EUR 717.60 EUR 358.80 EUR 253.20 EUR	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Ankyrin 1, Erythrocytic (ANK1)
ANK1 Protein Vector (Human) (pPB-His-MBP)
PV321786	ABM	500 ng	394.8 EUR
ANK1 Protein Vector (Human) (pPB-His-GST)
PV321787	ABM	500 ng	394.8 EUR
ANK1 Protein Vector (Rat) (pPB-C-His)
PV253566	ABM	500 ng	3423.6 EUR
ANK1 Protein Vector (Rat) (pPB-N-His)
PV253567	ABM	500 ng	3423.6 EUR
ANK1 Protein Vector (Rat) (pPM-C-HA)
PV253568	ABM	500 ng	3423.6 EUR
ANK1 Protein Vector (Rat) (pPM-C-His)
PV253569	ABM	500 ng	3423.6 EUR
ANK1 3'UTR Luciferase Stable Cell Line
TU000757	ABM	1.0 ml	1825.2 EUR
Ank1 3'UTR Luciferase Stable Cell Line
TU200614	ABM	1.0 ml	Ask for price
Ank1 3'UTR GFP Stable Cell Line
TU250614	ABM	1.0 ml	Ask for price
Ank1 3'UTR GFP Stable Cell Line
TU151791	ABM	1.0 ml	Ask for price
Ank1 3'UTR Luciferase Stable Cell Line
TU101791	ABM	1.0 ml	Ask for price
ANK1 3'UTR GFP Stable Cell Line
TU050757	ABM	1.0 ml	1825.2 EUR
ANK1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV669001	ABM	1.0 ug DNA	3190.8 EUR
ANK1 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV669005	ABM	1.0 ug DNA	3190.8 EUR
ANK1 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV669006	ABM	1.0 ug DNA	3190.8 EUR
Ank1 ELISA Kit| Mouse Ankyrin-1 ELISA Kit
EF014208	Lifescience Market	96 Tests	826.8 EUR
ANK1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
LV707337	ABM	1.0 ug DNA	379.2 EUR
ANK1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV707341	ABM	1.0 ug DNA	379.2 EUR
ANK1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV707342	ABM	1.0 ug DNA	379.2 EUR
Ankyrin 1, Erythrocytic (ANK1) Polyclonal Antibody (Human), APC
4-PAB633Hu01-APC	Cloud-Clone	399.60 EUR 3735.60 EUR 1046.40 EUR 507.60 EUR 258.00 EUR	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Ankyrin 1, Erythrocytic (ANK1). This antibody is labeled with APC.
Ankyrin 1, Erythrocytic (ANK1) Polyclonal Antibody (Human), Biotinylated
4-PAB633Hu01-Biotin	Cloud-Clone	363.60 EUR 2809.20 EUR 836.40 EUR 442.80 EUR 259.20 EUR	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Ankyrin 1, Erythrocytic (ANK1). This antibody is labeled with Biotin.
Ankyrin 1, Erythrocytic (ANK1) Polyclonal Antibody (Human), Cy3
4-PAB633Hu01-Cy3	Cloud-Clone	483.60 EUR 4930.80 EUR 1345.20 EUR 627.60 EUR 294.00 EUR	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Ankyrin 1, Erythrocytic (ANK1). This antibody is labeled with Cy3.
Ankyrin 1, Erythrocytic (ANK1) Polyclonal Antibody (Human), FITC
4-PAB633Hu01-FITC	Cloud-Clone	344.40 EUR 3012.00 EUR 860.40 EUR 430.80 EUR 230.40 EUR	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Ankyrin 1, Erythrocytic (ANK1). This antibody is labeled with FITC.
Ankyrin 1, Erythrocytic (ANK1) Polyclonal Antibody (Human), HRP
4-PAB633Hu01-HRP	Cloud-Clone	366.00 EUR 3256.80 EUR 926.40 EUR 459.60 EUR 243.60 EUR	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Ankyrin 1, Erythrocytic (ANK1). This antibody is labeled with HRP.
Ankyrin 1, Erythrocytic (ANK1) Polyclonal Antibody (Human), PE
4-PAB633Hu01-PE	Cloud-Clone	344.40 EUR 3012.00 EUR 860.40 EUR 430.80 EUR 230.40 EUR	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Ankyrin 1, Erythrocytic (ANK1). This antibody is labeled with PE.
Ankyrin 1, Erythrocytic (ANK1) Polyclonal Antibody (Human), APC-Cy7
4-PAB633Hu01-APC-Cy7	Cloud-Clone	656.40 EUR 7327.20 EUR 1948.80 EUR 872.40 EUR 372.00 EUR	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Ankyrin 1, Erythrocytic (ANK1). This antibody is labeled with APC-Cy7.
ANK1 Protein Vector (Human) (pPM-N-D-C-HA)
PV321788	ABM	500 ng	662.4 EUR
ANK1 Protein Vector (Human) (pPM-N-D-C-His)
PV321789	ABM	500 ng	662.4 EUR
Ank1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K3878005	ABM	3 x 1.0 ug	451.2 EUR
ANK1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K0086305	ABM	3 x 1.0 ug	451.2 EUR
Ank1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K6307305	ABM	3 x 1.0 ug	451.2 EUR
ANK1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV669002	ABM	1.0 ug DNA	3190.8 EUR
ANK1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV669003	ABM	1.0 ug DNA	3260.4 EUR
ANK1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV669004	ABM	1.0 ug DNA	3260.4 EUR
Ank1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K3878006	ABM	1.0 ug DNA	200.4 EUR
Ank1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K3878007	ABM	1.0 ug DNA	200.4 EUR
Ank1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K3878008	ABM	1.0 ug DNA	200.4 EUR
ANK1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K0086306	ABM	1.0 ug DNA	200.4 EUR
ANK1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K0086307	ABM	1.0 ug DNA	200.4 EUR
ANK1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K0086308	ABM	1.0 ug DNA	200.4 EUR
Ank1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)
K6307306	ABM	1.0 ug DNA	200.4 EUR
Ank1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)
K6307307	ABM	1.0 ug DNA	200.4 EUR
Ank1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)
K6307308	ABM	1.0 ug DNA	200.4 EUR
ANK1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV707338	ABM	1.0 ug DNA	379.2 EUR
ANK1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV707339	ABM	1.0 ug DNA	448.8 EUR
ANK1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
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We apply this mannequin to a single cluster of inositol trisphosphate (IP 3) receptor channels and discover additional proof for the outcomes introduced in earlier work: a single cluster could also be succesful of producing completely different calcium launch sorts, the place long-lasting occasions are accompanied by unbinding of IP Three from the receptor (Rückl et al., PLoS Comput. Biol. 11, e1003965 (2015)).

Finally, we present the practicability of the mannequin in a grid of 64 clusters which is computationally intractable with earlier high-resolution fashions. Here long-lasting occasions can lead to synchronized oscillations and waves, whereas brief occasions keep localized. The frequency of calcium releases in addition to their coherence can thereby be regulated by the amplitude of IP Three stimulation. Finally the mannequin permits for a brand new rationalization of oscillating [IP 3], which isn’t based mostly on metabolic manufacturing and degradation of IP 3.