Excitation-contraction (EC) coupling in skeletal muscle requires purposeful and mechanical coupling between L-type voltage-gated calcium channels (CaV1.1) and the ryanodine receptor (RyR1). Recently, STAC3 was recognized as an important protein for EC coupling and is an element of a gaggle of three proteins that may bind and modulate L-type voltage-gated calcium channels.
Here, we report crystal constructions of tandem-SH3 domains of completely different STAC isoforms up to 1.2-Å decision. These type a inflexible interplay by means of a conserved interdomain interface. We determine the linker connecting transmembrane repeats II and III in two completely different CaV isoforms as a binding website for the SH3 domains and report a crystal construction of the complicated with the STAC2 isoform.
The interplay website contains the placement for a illness variant in STAC3 that has been linked to Native American myopathy (NAM). Introducing the mutation doesn’t trigger misfolding of the SH3 domains, however abolishes the interplay. Disruption of the interplay through mutations within the II-III loop perturbs skeletal muscle EC coupling, however preserves the flexibility of STAC3 to decelerate inactivation of CaV1.2.
Calcium waves in a grid of clustered channels with synchronous IP3 binding and unbinding.
Calcium indicators in cells happen at a number of spatial scales and variable temporal length. However, a bodily rationalization for transitions between long-lasting international oscillations and localized short-term elevations (puffs) of cytoplasmic Ca2+ remains to be missing.
Here we introduce a phenomenological, coarse-grained mannequin for the calcium variable, which is represented by bizarre differential equations. Due to its small quantity of parameters, and its simplicity, this mannequin permits us to numerically research the interaction of multi-scale calcium concentrations with stochastic ion channel gating dynamics even in bigger techniques.
ANK1 Peptide |
46-725P |
ProSci |
0.1 mg |
EUR 338 |
Description: Ankyrin 1 / ANK1 Peptide |
ANK1 cloning plasmid |
CSB-CL001720HU1-10ug |
Cusabio |
10ug |
EUR 233 |
|
Description: A cloning plasmid for the ANK1 gene. |
ANK1 cloning plasmid |
CSB-CL001720HU2-10ug |
Cusabio |
10ug |
EUR 233 |
|
Description: A cloning plasmid for the ANK1 gene. |
Ankyrin 1 (ANK1) Antibody |
abx037600-100ug |
Abbexa |
100 ug |
EUR 391 |
|
Ankyrin 1 (ANK1) Antibody |
abx430864-200ul |
Abbexa |
200 ul |
EUR 286 |
|
Ankyrin R (ANK1) Antibody |
abx445096-100ug |
Abbexa |
100 ug |
EUR 523 |
|
Human ANK1 shRNA Plasmid |
20-abx950199 |
Abbexa |
|
|
|
Mouse ANK1 shRNA Plasmid |
20-abx969140 |
Abbexa |
|
|
|
Ankyrin 1, Erythrocytic (ANK1) Antibody |
20-abx128802 |
Abbexa |
-
EUR 411.00
-
EUR 133.00
-
EUR 1149.00
-
EUR 565.00
-
EUR 314.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Ankyrin R (ANK1) Antibody (ALP) |
abx442493-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (APC) |
abx442774-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (Biotin) |
abx443054-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (FITC) |
abx443334-100ug |
Abbexa |
100 ug |
EUR 565 |
|
Ankyrin R (ANK1) Antibody (HRP) |
abx443615-100ug |
Abbexa |
100 ug |
EUR 565 |
|
Ankyrin R (ANK1) Antibody (PerCP) |
abx444177-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (RPE) |
abx444458-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (Streptavidin) |
abx444739-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Polyclonal ANK1 antibody - middle region |
APR00810G |
Leading Biology |
0.05mg |
EUR 528 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ANK1 - middle region. This antibody is tested and proven to work in the following applications: |
Recombinant Ankyrin 1, Erythrocytic (ANK1) |
4-RPB633Hu01 |
Cloud-Clone |
-
EUR 476.32
-
EUR 230.00
-
EUR 1511.20
-
EUR 570.40
-
EUR 1040.80
-
EUR 382.00
-
EUR 3628.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Human Ankyrin 1, Erythrocytic expressed in: E.coli |
ANK1 ORF Vector (Human) (pORF) |
ORF000405 |
ABM |
1.0 ug DNA |
EUR 95 |
ANK1 ORF Vector (Human) (pORF) |
ORF000406 |
ABM |
1.0 ug DNA |
EUR 95 |
Ank1 ORF Vector (Rat) (pORF) |
ORF063392 |
ABM |
1.0 ug DNA |
EUR 2080 |
Ank1 ORF Vector (Mouse) (pORF) |
ORF038594 |
ABM |
1.0 ug DNA |
EUR 1572 |
Ank1 ORF Vector (Mouse) (pORF) |
ORF038595 |
ABM |
1.0 ug DNA |
EUR 1572 |
Human Ankyrin 1 (ANK1) ELISA Kit |
abx384586-96tests |
Abbexa |
96 tests |
EUR 911 |
|
Mouse Ankyrin 1 (ANK1) ELISA Kit |
abx388583-96tests |
Abbexa |
96 tests |
EUR 911 |
|
Ankyrin R (ANK1) Antibody (ATTO 390) |
abx440245-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (ATTO 488) |
abx440526-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (ATTO 565) |
abx440807-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (ATTO 594) |
abx441088-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (ATTO 633) |
abx441369-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (ATTO 655) |
abx441650-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (ATTO 680) |
abx441931-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Ankyrin R (ANK1) Antibody (ATTO 700) |
abx442212-100ug |
Abbexa |
100 ug |
EUR 578 |
|
Human Ankyrin 1, Erythrocytic (ANK1) Protein |
20-abx168126 |
Abbexa |
-
EUR 662.00
-
EUR 272.00
-
EUR 2040.00
-
EUR 787.00
-
EUR 481.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
ANK1 sgRNA CRISPR Lentivector set (Human) |
K0086301 |
ABM |
3 x 1.0 ug |
EUR 339 |
Ank1 sgRNA CRISPR Lentivector set (Mouse) |
K3878001 |
ABM |
3 x 1.0 ug |
EUR 339 |
Ank1 sgRNA CRISPR Lentivector set (Rat) |
K6307301 |
ABM |
3 x 1.0 ug |
EUR 339 |
Rat Ankyrin 1(ANK1) ELISA kit |
E02A1448-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Rat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Ankyrin 1(ANK1) ELISA kit |
E02A1448-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Rat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Ankyrin 1(ANK1) ELISA kit |
E02A1448-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Rat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Ankyrin 1(ANK1) ELISA kit |
E06A1448-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Ankyrin 1(ANK1) ELISA kit |
E06A1448-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Ankyrin 1(ANK1) ELISA kit |
E06A1448-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Ankyrin 1(ANK1) ELISA kit |
E08A1448-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Ankyrin 1(ANK1) ELISA kit |
E08A1448-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Ankyrin 1(ANK1) ELISA kit |
E08A1448-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Ankyrin 1(ANK1) ELISA kit |
E04A1448-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Ankyrin 1(ANK1) ELISA kit |
E04A1448-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Ankyrin 1(ANK1) ELISA kit |
E04A1448-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Ankyrin 1(ANK1) ELISA kit |
E09A1448-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Ankyrin 1(ANK1) ELISA kit |
E09A1448-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Ankyrin 1(ANK1) ELISA kit |
E09A1448-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Ankyrin 1(ANK1) ELISA kit |
E03A1448-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Ankyrin 1(ANK1) ELISA kit |
E03A1448-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Ankyrin 1(ANK1) ELISA kit |
E03A1448-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Ankyrin 1(ANK1) ELISA kit |
E07A1448-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Ankyrin 1(ANK1) ELISA kit |
E07A1448-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Ankyrin 1(ANK1) ELISA kit |
E07A1448-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Ankyrin 1(ANK1) ELISA kit |
E01A1448-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Human Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Ankyrin 1(ANK1) ELISA kit |
E01A1448-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Human Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Ankyrin 1(ANK1) ELISA kit |
E01A1448-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Human Ankyrin 1(ANK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Ankyrin R (ANK1) Antibody (PE/ATTO 594) |
abx443896-100ug |
Abbexa |
100 ug |
EUR 592 |
|
Polyclonal Ankyrin 1 / ANK1 Antibody (C-Term) |
APG00534G |
Leading Biology |
0.1mg |
EUR 484 |
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Ankyrin 1 / ANK1 (C-Term). This antibody is tested and proven to work in the following applications: |
Ankyrin 1, Erythrocytic (ANK1) Polyclonal Antibody (Human) |
4-PAB633Hu01 |
Cloud-Clone |
-
EUR 239.00
-
EUR 2391.00
-
EUR 598.00
-
EUR 299.00
-
EUR 211.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Ankyrin 1, Erythrocytic (ANK1) |
ANK1 sgRNA CRISPR Lentivector (Human) (Target 1) |
K0086302 |
ABM |
1.0 ug DNA |
EUR 154 |
ANK1 sgRNA CRISPR Lentivector (Human) (Target 2) |
K0086303 |
ABM |
1.0 ug DNA |
EUR 154 |
ANK1 sgRNA CRISPR Lentivector (Human) (Target 3) |
K0086304 |
ABM |
1.0 ug DNA |
EUR 154 |
Ank1 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
K3878002 |
ABM |
1.0 ug DNA |
EUR 154 |
Ank1 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
K3878003 |
ABM |
1.0 ug DNA |
EUR 154 |
Ank1 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
K3878004 |
ABM |
1.0 ug DNA |
EUR 154 |
Ank1 sgRNA CRISPR Lentivector (Rat) (Target 1) |
K6307302 |
ABM |
1.0 ug DNA |
EUR 154 |
Ank1 sgRNA CRISPR Lentivector (Rat) (Target 2) |
K6307303 |
ABM |
1.0 ug DNA |
EUR 154 |
Ank1 sgRNA CRISPR Lentivector (Rat) (Target 3) |
K6307304 |
ABM |
1.0 ug DNA |
EUR 154 |
ANK1 3'UTR Luciferase Stable Cell Line |
TU000757 |
ABM |
1.0 ml |
EUR 1521 |
Ank1 3'UTR GFP Stable Cell Line |
TU250614 |
ABM |
1.0 ml |
Ask for price |
Ank1 3'UTR Luciferase Stable Cell Line |
TU200614 |
ABM |
1.0 ml |
Ask for price |
Ank1 3'UTR GFP Stable Cell Line |
TU151791 |
ABM |
1.0 ml |
Ask for price |
ANK1 3'UTR GFP Stable Cell Line |
TU050757 |
ABM |
1.0 ml |
EUR 1521 |
Ank1 3'UTR Luciferase Stable Cell Line |
TU101791 |
ABM |
1.0 ml |
Ask for price |
ANK1 Protein Vector (Human) (pPB-C-His) |
PV001617 |
ABM |
500 ng |
EUR 329 |
ANK1 Protein Vector (Human) (pPB-N-His) |
PV001618 |
ABM |
500 ng |
EUR 329 |
ANK1 Protein Vector (Human) (pPM-C-HA) |
PV001619 |
ABM |
500 ng |
EUR 329 |
ANK1 Protein Vector (Human) (pPM-C-His) |
PV001620 |
ABM |
500 ng |
EUR 329 |
ANK1 Protein Vector (Human) (pPB-C-His) |
PV001621 |
ABM |
500 ng |
EUR 329 |
ANK1 Protein Vector (Human) (pPB-N-His) |
PV001622 |
ABM |
500 ng |
EUR 329 |
ANK1 Protein Vector (Human) (pPM-C-HA) |
PV001623 |
ABM |
500 ng |
EUR 329 |
ANK1 Protein Vector (Human) (pPM-C-His) |
PV001624 |
ABM |
500 ng |
EUR 329 |
ANK1 Protein Vector (Human) (pPB-His-MBP) |
PV321786 |
ABM |
500 ng |
EUR 329 |
ANK1 Protein Vector (Human) (pPB-His-GST) |
PV321787 |
ABM |
500 ng |
EUR 329 |
ANK1 Protein Vector (Rat) (pPB-C-His) |
PV253566 |
ABM |
500 ng |
EUR 2853 |
ANK1 Protein Vector (Rat) (pPB-N-His) |
PV253567 |
ABM |
500 ng |
EUR 2853 |
ANK1 Protein Vector (Rat) (pPM-C-HA) |
PV253568 |
ABM |
500 ng |
EUR 2853 |
ANK1 Protein Vector (Rat) (pPM-C-His) |
PV253569 |
ABM |
500 ng |
EUR 2853 |
We apply this mannequin to a single cluster of inositol trisphosphate (IP 3) receptor channels and discover additional proof for the outcomes introduced in earlier work: a single cluster could also be succesful of producing completely different calcium launch sorts, the place long-lasting occasions are accompanied by unbinding of IP Three from the receptor (Rückl et al., PLoS Comput. Biol. 11, e1003965 (2015)).
Finally, we present the practicability of the mannequin in a grid of 64 clusters which is computationally intractable with earlier high-resolution fashions. Here long-lasting occasions can lead to synchronized oscillations and waves, whereas brief occasions keep localized. The frequency of calcium releases in addition to their coherence can thereby be regulated by the amplitude of IP Three stimulation. Finally the mannequin permits for a brand new rationalization of oscillating [IP 3], which isn’t based mostly on metabolic manufacturing and degradation of IP 3.