●siRNA gene silencers are
composed of three to five pairs of 19-25nt (nucleotide), and the concentration of 10μM can provide 50-100 transfections. ● shRNA plasmid DNA uses Hi promote to make shRNA stable and continuous performance. With puromycin resistant, antibiotics can be used for screening ● shRNA Lentiviral particles use viral infection to enter cells
A1: shRNA Lentiviral Particles use viral infection to enter cells. It is recommended to use in primary cells that are not easily transfected.
Q2: Are the siRNA and shRNA sequences the same?
Q3 : How to confirm the effect of transfection?
A3: The green fluorescence of copGFP plasmid (sc-108083) and copGFP Lentiviral Particles (sc-108084) can be used to confirm transfection or transduction efficiency.
Q4: shRNA plasmaid catalog number (h) and (h2 The difference between )?
A4: (h) and (h2) are used to silence the same gene, but the designed sequence is different.
Q5: Can the sequence of siRNA or shRNA be provided?
A5: Yes, just provide the lot number after ordering. !
Q6: Can lentiviral vector plasmid be amplified by itself?
Q7: shRNA contains 3-5 plastids? Can it be purchased separately?
A7: The original factory currently only provides siRNA sequences to be purchased separately
|Catalog #||Product name|
|sc-29528||siRNA Transfection Reagent|
|sc-36868||siRNA Transfection Medium|
|sc-29527||siRNA Dilution Buffer|
|sc-45064||siRNA Reagent System|
|sc-108061||shRNA Plasmid Transfection Reagent|
|sc-108062||shRNA Plasmid Transfection Medium|
|sc-108060||Control shRNA Plasmid-A|
|sc-108083||copGFP Control Plasmid|
|sc-108080||Control shRNA Lentiviral Particles|
|sc-108084||copGFP Control Lentiviral Particles|