siRNA & shRNA Gene Silencers

RNAi (RNA interference): The combination of small fragments of nucleotide molecules with complementary mRNAs leads to degradation of the mRNA and gene knockdown. The Santa Cruz RNAi system includes siRNA, shRNA Plasmid and shRNA Lentiviral particles. The product line covers more than 99% mouse and human protein-coding genes.

●siRNA gene silencers are
composed of three to five pairs of 19-25nt (nucleotide), and the concentration of 10μM can provide 50-100 transfections. ● shRNA plasmid DNA uses Hi promote to make shRNA stable and continuous performance. With puromycin resistant, antibiotics can be used for screening ● shRNA Lentiviral particles use viral infection to enter cells

Common Q&A
Q1: What are the main differences between shRNA Lentiviral Particles and shRNA Plasmids?
A1: shRNA Lentiviral Particles use viral infection to enter cells. It is recommended to use in primary cells that are not easily transfected.

Q2: Are the siRNA and shRNA sequences the same?
A2: Yes

Q3 : How to confirm the effect of transfection?
A3: The green fluorescence of copGFP plasmid (sc-108083) and copGFP Lentiviral Particles (sc-108084) can be used to confirm transfection or transduction efficiency.

Q4: shRNA plasmaid catalog number (h) and (h2 The difference between )?
A4: (h) and (h2) are used to silence the same gene, but the designed sequence is different.

Q5: Can the sequence of siRNA or shRNA be provided?
A5: Yes, just provide the lot number after ordering. !

Q6: Can lentiviral vector plasmid be amplified by itself?
A6: No

Q7: shRNA contains 3-5 plastids? Can it be purchased separately?
A7: The original factory currently only provides siRNA sequences to be purchased separately

Transfection Reagents area
Catalog # Product name
sc-29528 siRNA Transfection Reagent
sc-36868 siRNA Transfection Medium
sc-29527 siRNA Dilution Buffer
sc-45064 siRNA Reagent System
sc-37007 Control siRNA-A
sc-108061 shRNA Plasmid Transfection Reagent
sc-108062 shRNA Plasmid Transfection Medium
sc-108071 Puromycin dihydrochloride
sc-108060 Control shRNA Plasmid-A
sc-108083 copGFP Control Plasmid
sc-134220 Polybrene
sc-108080 Control shRNA Lentiviral Particles
sc-108084 copGFP Control Lentiviral Particles

 

 

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