ACD RNAscope ISH-IHC
2 wishes to satisfy
Transcriptomics & Proteomics
🌟Overcoming the problems of low sensitivity and low specificity of traditional ISH, RNAscope has the advantages of high sensitivity, high specificity, time-saving and easy operation, allowing RNAscope ISH-IHC to get better results
🌟 can be applied to: Immuno-oncology, Neurobiology , Cell, Gene Therapy & IHC Validation
Three precautions before implementing Dual ISH-IHC/IF
⭐Confirm that RNAscope ISH can get good results⭐Confirm
that antibodies can get good results in IHC /
IF⭐ Since the pre-processing of RNAscope will use Protease, it is recommended to use Protease for pre-processing before testing IHC/IF experiments to confirm antibodies and proteins The binding position will not be affected, the IHC/IF signal has not weakened, and the background value has not increased (as shown below).
(B) Detect the RNA of TGFB and FOXP3 and the T cell Marker CD4 protein.
RNAscope HIplex + IHC
Dual ISH-IHC / IF can detect different neuronal subtypes and detect the expression of circRNA splice variants in specific cells.
RNAscope HiPlex can detect up to 12 target RNAs; the figure simultaneously detects the performance of Drd1 + and Drd2 + striatal neuron subtypes, neuron Marker NeuN protein (white) and circRNA splice variants.